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In order to examine the effect of TiO₂ on photosterilization of microorganism, proper content of TiO₂, illumination time, wave length specificity and cell concentration were investigated for photosterilization of E. coli, B. subtilis and S. cerevisiae. The amount of TiO₂ for photosterilization of E. coli was effective in the range of 5∼20 ㎎%. The sterilization time was reduced when the low wave length below 400 ㎚ was not cut off by glass, but the catarizing effect of TiO₂ was similar to overall wave length. The photosterilization effects by TiO₂ addition was recognized among S. cerevisiae, E. coli and B. subtilis in that order. The photosterilization time of S. cerevisiae was considerably reduced at 10⁴/㎖ cell concentration compared to 10^5/㎖.
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